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Léčba autoimunitních onemocnění helmintoterapií
STRANKMÜLLEROVÁ, Zuzana
This bachelor thesis will discuss the impact of gastrointestinal helminths on the human immune system concentrating especially on their therapeutical potency in the treatment of autoimmune diseases. Epidemiological studies show a significant difference between developing and developed countries. This inequality gave rise to the two hypotheses: the Hygiene Hypothesis and the Old Friend Hypothesis. These theories mention the fact that exposure to the parasites may lower the risk of developing autoimmune diseases. Anti-inflammatory products of helminths modulate the immune system to induce Th2 response (with rising levels of anti-inflammatory cytokines e.g. IL-4, IL-10 a TGF-). Therefore, helminths or their molecules can cure autoimmune inflammatory diseases or even diseases of civilisation.
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Sledování imunomodulačních účinků extraktů z helminta na makrofágové buněčné kultuře
ŘEŽÁBKOVÁ, Lucie
The main aim of the present study was to determine the immunomodulatory effects of extracts obtained from the commensal helminth, Hymenolepis diminuta, on in vitro rat macrophages. Here, I tested two types of crude extracts derived from various tapeworm's life stages (larval stages, adults) and excretory/secretory products (ES-products). The in vitro inflammatory model was induced by bacterial lipopolysaccharides (LPS) to compare the results with normal state of macrophages. To monitoring immune response, I analysed the relative gene expressions of several cytokines TNF, IL-10, IL-1; signal transducer and activator of transcription STAT6 as well as IL-17re using by qPCR. The presence of adult extracts caused no obvious immune response of macrophages. The larval extract and ES-products induced an inflammatory response. All three types of compounds derived from H. diminuta reduced the inflammation of macrophages influenced by LPS.
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Benigní helmint Hymenolepis diminuta pozitivně ovlivňuje chemicky vyvolanou kolitidu u potkaního modelu
LEVÁ, Jana
In this study is examined the protective effect of different life cycles stages of rat tapeworm Hymenolepis diminuta against dinitrobenzene sulphonic acid (DNBS) induced colitis in rat model. The clinical health of rats, gut microbiota and systemic inflammation was analysed. For determination of the level of inflammation and type 2 immune response of rat model was used relative gene expression of TNF, IL-1, IL-4, IL-13 and IL-10 using real-time PCR. Our results showed that colonization by immature life stages of H. diminuta before the induction of DNBS colitis reduced clinical symptoms and inflammation in rat model but did not protect rats against colitis. Type 2 immune response was detected by an increase in gene expression of IL-4, IL-10 and IL-13. The gut microbiota does not substantial role in H. diminuta-mediated protection.
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